ABSTRACT
AIM To prepare the silkworm pupa polysaccharide capsules.METHODS The crude silkworm pupa polysaccharide was extracted by alkali-assisted ultrasonic wave.After deproteinization and decolorization,it was dialysed,and then isolated and purified by SephadexG-150 column.Single factor test was applied to optimizing three influencing factors including ethanol concentration,soild-liquid ratio and drying temperature.The polysaccharide powder's angle of repose,bulk density and critical relative humidity were determined,after which the water content,load difference and disintegration time of obtained capsules were investigated.RESULTS The optimal conditions were determined to be 90％ for ethanol concentration,1 ∶ 1 for soild-liquid ratio,and 60 ℃ for drying temperature.For polysaccharide powder,the average angle of repose was 37.74℃,the average bulk density was 0.51 g/mL,and the critical relative humidity was 63％.With 6.87％ for water content,0.132 3 g for average load,and 12.12 min for average disintegration time,the capsules were qualified according to standard requirements of Chinese Pharmacopoeia.CONCLUSION The good formability of silkworm pupa polysaccharide capsules demonstrates that this simple,stable and controllable technology can provide an experimental basis for the development of related drugs.